Review: Gene of 16S rRNA is the gold standard to diagnose bacteria
Abstract
The success of the biochemical tests depends on the purity of the culture, and this requires special preparations for obtaining, transporting and purifying of sample, use of selective media, duration of incubation, temperatures and others and all this takes time and effort.It may require sending slow-growing pathogens to laboratories that provide the requirements for their growth, but they may be far from the source of their collection, and thus it takes a long time to diagnose pathogen. Also, biochemical tests reflect the activity of the gene responsible for the production of a particular substance, and this gene may be present, but it does not work, and therefore the result may be a misdiagnosis. Gene of 16S rRNA is found in all bacteria, and it contains stable regions through which bacteria can be identified, and other variable regions through which bacterial species can be distinguished. As well as, diagnosis of 16S rRNA gene does not require the requirements that are required by biochemical tests for the diagnosis of bacteria, and mutations that occur in this gene do not affect its basic function, which is diagnosis, so 16S rRNA gene is the gold standard for bacterial diagnosis. In principle, it is possible to rely on phenotypic characteristics to diagnose bacteria, but confirmatory diagnosis and registration of new species is done through 16S rRNA gene using polymerase chain reaction.
Keywords:
Biochemical tests, Isolation of bacteria, Identification of bacteria, 16S rRNA gene, Polymerase chain reaction, Agarose gel electrophoresis, Sequence of geneDOI
https://doi.org/10.37022/wjcmpr.v5i4.283References
1. Abd al-Razzaq A A and Hamim SS 2019. Serological and molecular detection of Staphylococcusaureus isolated from UTI patients. University of Thi-Qar Journal 14(4): 17.
2. Abd al-Rida AA, Yasir I K, Swadi A J 2019. The bacteriological study of Acinetobacterbaumannii isolated from burn patients in al-Nasiriyah city-Iraq. University of Thi-Qar Journal 14(4):26.
3. Aguilera‑Arreola MG, Martínez‑Peña MD, Hernández‑Martínez F, Enriques SRJ, Verdín BR, Majalca‑Martínez C, Castro‑Escarpulli G, Albarrán‑Fernández E and CeSerrano‑López S C 2016.Cultivation‑independent approach for the direct detection of bacteria in human clinical specimens as a tool for analyzing culture‑negative samples: a prospective study.SpringerPlus,5:332
4. Al-Hilali R and Al-Mozan H 2023. Isolation and identification of Gram negative bacteria that cause diarrhea. University of Thi-Qar Journal of Science 10(1):1-6.
5. AL-Joda BMS and Jasim AH 2021. Biochemical Testing Revision For Identification Several Kinds of Bacteria. Journal of University of Babylon for Pure and Applied Science, 29(2): 168-176.
6. Al-Mozan HDK 2021. Molecular detection of enterotoxins genes in Staphylococcusaureus isolated from clinical and dairy samples. A thesis submitted to the college of science/ University of Basrah:1-131.
7. Al-Mozan HDK and Al-Amara SSM 2020. The nuc gene as a potent source for Staphylococcus aureus to resist thermal processes. International Journal of Pharmaceutical Research 12(2): 4614-4617.
8. Al-Yousif N A 2022. Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq. Biodiversitas Journal of Biological Diversity 23(2):679-686.
9. Aminollah BI, and Supriyanto A (2016). Isolasi dan Identifikasi Bakteri Patogen Escherichia coli Dan Salmonella sp pada Kotoran Kelelawar di Gua Pongangan, Gresik dan Gudang Talun Bojonegoro, Jawa Timur, in: Prosiding Seminar Nasional. Surabaya. Available at: http://biologi.fst.unair.ac.id/wp-content/uploads/2016/10/Aminollah.pdf
10. Amutha K, and Kokila V 2014. PCR Amplification, Sequencing of 16S rRNA Genes with Universal Primers and Phylogenetic Analysis of Pseudomonas aeruginosa. International Journal of Science and Research, 3(8):257-261.
11. Anahtar MN, Bowman BA, Kwon DS 2016. Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization. Journal of Visualized Experiments, (110), e53939, doi:10.3791/53939
12. Aryal, S. (2018a). Methyl Red (MR) Test - Principle, Procedure and Result Interpretation.https://microbiologyinfo.com/methyl-red-mr-test-principle-procedure-and-result-interpretation/
13. Aryal, S. (2018b). Voges-Proskauer (VP) Test - Principle, Reagents, Procedure and Result.https://microbiologyinfo.com/voges-proskauer-vp-test-principle-reagents-procedure-and-result/
14. Ayeni FA, Gbarabon T, Andersen C, Nørskov-Lauritsen N. 2015. Comparison of identification and antimicrobial resistance pattern of Staphylococcus aureus isolated from Amassoma, Bayelsa state, Nigeria. African Health Sciences 15(4):1282-1288. DOI10. 4314/ ahs. v15i4. 30.
15. Badreh F, Jahanbin K, Khodadadi A, Zadeh AK, Sharifat M, Khayati M, Rashno M 2022. Homemade 100 Pair Base DNA Ladder Based on Bacterial Plasmid pMAL-C2X. Pharmacogenom Pharmacoproteomics, 14 (3): 1-4.
16. Bailen M, Bressa C, Larossa M, and Gonzales-Sontero R. 2020. Bioinformatic strategies to address limitations of 16rRNA short-read amplicons from different sequencing platforms. Journal of Microbiological Method, 169, 105811.
17. Beden MW and Mousa H M 2022. Bacteriuria with Unusual Uropathogens in Diabetics in Thi-Qar Province. University of Thi-QarJournal of Science 9(1):43-48.
18. Benson DA. et al. 2013. GenBank. Nucleic Acids Res. 41, D36–D42.
19. Burillo A and Bouza E 2014. Use of rapid diagnostic techniques in ICU patients with infections. BMC Infectious Diseases 14:1-12.
20. Cahyadi M i, Puruhita, Barido F H, and Hertanto B S 2017. Specific primer design of mitochondrial 12S rRNA for species identification in raw meats. IOP Conf. Series: Earth and Environmental Science 102,012038.doi:10.1088/17551315/102/1/012038
21. Ceyssens P, Glonti T, Kropinski NM, Lavigne R, Chanishvili N, Leonid Kulakov L, Nino Lashkhi N, Tediashvili M and Merabishvili M 2011. Phenotypic and genotypic variations within a single bacteriophage species. Virology Journal, 8: 134. http:// www. virologyj. com/ content/ 8/1/134
22. Chen L, Cai Y, Zhou G, Shi X, Su J, Chen G and Lin K 2014. Rapid Sanger Sequencing of the 16S rRNA Gene for Identification of Some Common Pathogens. PLoS One. 9(2): e88886 doi: 10.1371/journal.pone.0088886
23. Chiu CY and Miller SA 2019. Clinical metagenomics. Nat. Rev. Genet. 20: 341–355.
24. Clarridge JE 2004. Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases. CLINICAL MICROBIOLOGY REVIEWS 17(4): 840–862.
25. Das S, Dash HR, Mangwani N, Chakraborty J and Kumari S 2014. Understanding molecular identification and polyphasic taxonomic approaches for genetic relatedness and phylogenetic relationships of microorganisms. Journal of Microbiological Methods 103: 80–100.
26. Davis NM, Proctor DM, Holmes SP, Relman DA and Callahan BJ 2018. Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data. Microbiome, 6:226. https://doi. org/10. 1186/ s40168-018-0605-2
27. Desjardins RP, Conklin DS 2011. Microvolume quantitation of nucleic acids. Curr. Protoc. Mol. Biol. 93(1), 3J.
28. Devlynne S, Ondusko MD, Dawn Nolt, MD 2018.Staphylococcusaureus. Oregon Health & Science University, Portland, 39(6):287-298.
29. Dhahir, A.W. (2023). Genotypic Characterization of Bacteria isolated from intensive care units in Basrah hospitals. A thesis submitted to the council of college of science, university of Basrah.
30. Dickson R P et al. 2020. Lung microbiota predict clinical outcomes in critically Ill patients. Am. J. Respir. Crit. Care Med. 201(5), 555–563.
31. Edgar RC 2018. Updating the 97% identity threshold for 16S ribosomal RNA OTUs. Bioinformatics, 34(14),: 2371–2375. doi: 10.1093/bioinformatics/bty113
32. ELMA LL, ALBERTO F, DE OLIVEIRA JR, FILLIPE L.R, CARMO D,BERKOVA N, BARH D, PREETAM GHOSH P, and AZEVEDO V 2020. Bacteriocins as an alternative in the treatment of infections by Staphylococcus aureus. An Acad Bras Cienc , 92(2):1-17.
33. Ernande B and Dieckmann U 2004. The evolution of phenotypic plasticity in spatially structured environments: implications of intraspecific competition, plasticity costs and environmental characteristics. J . EVOL. B IOL. 17: 613–628.
34. Favrot C 2015. Polymerase chain reaction: advantages and drawbacks. Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-116536
35. Fernández-Barat L, López-Aladid R. and Torres A 2020. Reconsidering ventilator-associated pneumonia from a new dimension of the lung microbiome. EBioMedicine 60, 102995.
36. Gee JE, De BK, Levett PN, Whitney AM Novak RT and Popovic T 2004.Rapid Confirmatory Identification of Use of 16S rRNA Gene Sequencing for Brucella Isolates. J. Clin. Microbiol. 2004, 42(8):3649. DOI:10.1128/JCM.42.8.3649-3654.2004.
37. Ghannam MG, and Varacallo M 2023. Biochemistry, Polymerase Chain Reaction.National Library of Medicine, https://www.ncbi.nlm.nih.gov/books/NBK535453/
38. Gomez-Mestre I and Jovani R. 2013. A heuristic model on the role of plasticity in adaptive evolution: plasticity increases adaptation, population viability and genetic variation. Proceedings of the Royal Society B 280:20131869
39. Goswami K, Chen C, Xiaoli L, Eaton KA Dudley EG 2015. Coculture of Escherichiacoli O157: H7 with a Nanpathogenic E. coli strain increases toxin production and virulence in a germfree mouse model. Infection and Immunity 83(11): 4185- 4193.
40. Graham S, Marina PF, Blencowe A. 2019. Thermoresponsive polysaccharides and their thermoreversible physical hydrogel networks. Carbohydr. Polym. 207, 143–159.
41. Gu W, Miller S, Chiu CY 2019. Clinical Metagenomic Next- Generation Sequencing for Pathogen Detection.Annu. Rev. Pathol.14: 319–338.
42. Haines AM, Tobe S, Kobus HJ, Linacre A 2015. Properties of nucleic acid staining dyes used in gel electrophoresis. Electrophoresis 36(6): 941–944.
43. Hartline, R 2023.MICROBIOLOGY LABORATORY MANUAL. LabreTexts.https://LibreTexts.org
44. Heather JM, and Chain B 2015. The sequence of sequencers: The history of sequencing DNA. Genomics 107: 1–8. http://dx.doi.org/10.1016/j.ygeno.2015.11.003.
45. Heckmann CM and Paradisi F 2020. Looking Back: A Short History of the Discovery of Enzymes and How They Became Powerful Chemical Tools. ChemCatChem , 12: 6082–6102. doi.org/10.1002/cctc.202001107
46. Hemraj V, Diksha S and Avneet G 2013. A REVIEW ON COMMONLY USED BIOCHEMICAL TEST FOR BACTERIA. Innovare Journal of Life Science, 1(1):1-7.
47. Hendry AP 2015. Key questions on the role of phenotypic plasticity in eco evolutionary dynamics. Journal of Heredity 107:25–41.
48. Hunt K, Vacelet M and Jordan K 2017.Determination of Listeria monocytogenes numbers at less than 10 cfu/g. Irish Journal of Agricultural and Food Research,DOI: 10.1515/ijafr-2017-0004 IJAFR • 56(1) :25-30.
49. Igbeneghu O and Lamikanra A 2014. The bacteriological quality of different brands of bottled water available to consumers in Ile-Ife, south- western Nigeria. a BMC Research Notes 7:859.
50. Irwan JM, Anneza LH, Othman N, Husnul T and Alshalif AF 2016. Isolation and Identification of Concrete Environment Bacteria. IOP Conf. Series: Materials Science and Engineering 136, 012088 doi:10.1088 /1757-899X/136/1/012088
51. Ishino S, and Ishino Y 2014. DNA polymerases as useful reagents for biotechnology – the history of developmental research in the field, Front Microbiol. 2014; 5: 465. doi: 10.3389/fmicb.2014.00465
52. Jang J , Hur HG, Sadowsky MJ, Byappanahalli MN, Yan T and Ishii S 2017. Environmental Escherichia coli: ecology and public healthimplications—a review. Journal of Applied Microbiology, 123, 570—581.
53. Jeong T, Hong H, Kim MS, Song M, and Myung H 2023. Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria. Viruses, 15, 679. https://doi.org/10.3390/v15030679.
54. Jesumirhewe C J, Ogunlowo PO, Olley M, Springer B, Allerberger F and Ruppitsch W 2016. Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals. PeerJ, DOI 10.7717/peerj.2511
55. Jiang F, Xu X, Chen F, Weng H, Jun Chen J, Ru Y, Xiao Q and Xiao A 2023. Extraction, Modification and Biomedical Application of Agarose Hydrogels: A Review. Mar. Drugs , 21: 299 .https://doi.org/10.3390/md21050299
56. Joshi, M and Deshpande J.D 2011. POLYMERASE CHAIN REACTION: METHODS, PRINCIPLES AND APPLICATION. International Journal of Biomedical Research, 1(5): 81‐97.
57. Karnath L 2019. EDITORIAL Remembering Dr. Kary Mullis— Inventor of PCR. MOLECULAR FRONTIERS JOURNAL, 3(2).
58. Kaushal J, Mehandia S, Singh G, Raina A, Arya S K.2018. Catalase enzyme: Application in bioremediation and food industry. Biocatalysis and Agricultural Biotechnology, 16: 192-199.
59. Kchouk M, Gibrat J and Elloumi M 2017. Generations of Sequencing Technologies: From First to Next Generation. Biol Med, 9 (3): 1-8.
60. Kshikhundo R, and Itumhelo S 2016. Bacterial species identification. World News of Natural Sciences, 3: 26-38.
61. Lakshmi P, Bharadwaj A and Srivastava RK 2020.Molecular Detection and Identification of Bacteria in Urine Samplesof Asymptomatic and Symptomatic PregnantWomen by 16S Rrna Gene Sequencing.Arch Clin Infect Dis. 15(3) :e101136.doi:10.5812/archcid.101136.
62. Leraya M, Knowltonb N, Hoc S, Bryan N. Nguyenb BN, and Machidac RJ 2019. GenBank is a reliable resource for 21st century biodiversity research. ENVIRONMENTAL SCIENCES, 116(45): 22651–22656. www.pnas. org/cgi/ doi/ 10. 1073/pnas.1911714116
63. Li C and Arakawa T 2019. Agarose native gel electrophoresis of proteins. Int. J. Biol. Macromol, 140: 668–671.
64. Lindner MS, Renard BY 2013. Metagenomic abundance estimation and diagnostic testing on species level. Nucleic Acids Res4, 1(1):10–10.
65. Lira RB, Steinkuhler J, Knorr R.L, Dimova R, Riske KA 2016. Posing for a picture: Vesicle immobilization in agarose gel. Sci. Rep. 6: 25254.
66. Mai-Prochnow A, Clauson M, Hong, J and Murphy AB 2016. Gram positive and Gram negative bacteria differ in their sensitivity to cold plasma. Scientific Reports | 6:38610 | DOI:10.1038/srep38610
67. Mardis E, McCombie WR 2017. Library quantification: fluorometric quantitation of double-stranded or single-stranded DNA samples using the Qubit system. Cold Spring Harb. Protoc. (6),pdb.prot094730
68. Marx V 2016. PCR: the price of infidelity. Nature Methods. U.S. Food & Drug Administration, 13:475–479. DOI: 10.1038/nmeth.3868
69. Maurer FM, Christner M, Hentschke M, Rohde H 2017. Advances in rapid identification and susceptibility testing of bacteria in the clinical microbiology laboratory: implications for patient care and antimicrobial stewardship programs. Infectious Disease Reports 9:18-27.
70. Muktaf A Q 2022. Effect of hot aqueous and ethanolic extracts of two algae species (Phaeophyta) on some pathogenic bacteria isolated from patients. A thesis submitted to the college of science/ University of Thi-Qar:1-118.
71. Murren CJ, Auld JR, Callahan H, Ghalambor CK, Handelsman CA, Heskel MA, Kingsolver JG, Maclean HJ, Masel J, MaughanH, Pfennig DW, Relyea RA, Seiter S, Snell-Rood E, Steiner UK and Schlichting CD 2015. Constraints on the evolution of phenotypic plasticity: limits and costs of phenotype and plasticity. Heredity, 115, 293–301; doi: 10. 1038/ hdy. 2015.8
72. Nagai T, Shiba T, Komatsu K, Watanabe T, Nemoto T, Maekawa S, Kobayashi R, Matsumura S, Ohsugi Y, Katagiri S, Takeuchi Y, Iwata T 2023. Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and database. DOI: https://doi.org/10.21203/rs.3.rs-3139837/v1
73. Negahdaripour M and PharmD 2020. Fifty Years of Witnessing Biomedical Science Developments. Iran J Med Sci November, 45 (6).
74. Petti C A, Polage C R, Schreckenberger P (2005). The Role of 16S rRNA Gene Sequencing in Identification of Microorganisms Misidentified by Conventional Methods. JOURNAL OF CLINICAL MICROBIOLOGY 43 (12): 6123–6125.
75. Porter TM and Hajibabaei M 2018. Over 2.5 million COI sequences in GenBank and growing. PLoS One 13, e0200177
76. Public Health England. 2018. UK Standards for Microbiology Investigations. Issued by the Standards Unit, Public Health England, 4:12
77. Putria I, Jannaha SN and Purwantisaria S 2020. Isolation and characterization of lactic acid bacteria from Apis mellifera and their potential as antibacterial using in vitro test against growth of Listeria monocytogenes and Escherichia coli.NICHE Journal of Tropical Biology, 3(1): 26-34.
78. Qazangi MO and Jabr AH 2017. Molecular Biology. 1th Edition, Iraq, University of Baghdad – Science of Agriculture, 1-436.
79. Qazangi MOM 2017.Practical experiments in microbiology. 1th Edition, Iraq, University of Baghdad – Science of Agriculture : 1-138.
80. RASHEED MU, THAJUDDIN N. AHAMED P, TEKLEMARIAM Z, and JAMIL K 2014. ANTIMICROBIAL DRUG RESISTANCE IN STRAINS OF Escherichia coli ISOLATED FROM FOOD SOURCES. Med. Trop. Sao Paulo, 56(4): 341-6.
81. Reddy PR and Raju N (2012). Gel-Electrophoresis and Its Applications, Gel Electrophoresis - Principles and Basics, Sameh Magdeldin , ISBN: 978-953-51-0458-2, InTech, Available from: http://www.intechopen.com/books/gel-electrophoresis-principles-and-basics/ gelelectrophoresi-andits applications
82. Rizal NSM., Ramli R., Periyasamy PR, Tan TL, Nathan S, Saw SH, Khor BY 2020. Advantages and Limitations of 16S rRNA Next-Generation Sequencing for Pathogen Identification in the Diagnostic Microbiology Laboratory: Perspectives from a Middle-Income Country. Diagnostics 816: 1-19. doi:10.3390/diagnostics10100816
83. Romaniuk J A. H. and Cegelski L 2015. Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR. the Royal Society, http://dx.doi.org/10.1098/rstb.2015.0024
84. Shanmugaraj C, Anokhe A, and Kalia V 2021. Determination of Fermentation Pathway by Methyl Red and Voges Proskauer (MRVP) Test. AgriCos e-Newsletter,2(11):41-43.
85. Shepherd M.J, Horton J.S, and Taylor T.B 2022. A Near-Deterministic Mutational Hotspot in Pseudomonas fluorescens Is Constructed by Multiple Interacting Genomic Features. Mol. Biol. Evol. 39(6):1-7. https://doi.org/10.1093/molbev/msac132
86. Silhavy TJ, Kahne D, Walker S 2010. The bacterial cell envelope. Cold Spring Harbor Perspectives in Biology 2, a000414. doi: 10.1101/cshperspect.a000414.
87. Srinivasan R, Karaoz U, Volegova M MacKichan J, Kato- Maeda M, Miller S, Nadarajan R, Brodie EL and Lynch SV 2015. Use of 16S rRNA Gene for Identification of a Broad Range of Clinically Relevant Bacterial Pathogens. PLOS ONE | DOI:10.1371/journal.pone.0117617
88. Strasser B J 2008. GenBank—Natural history in the 21st century? Science 322: 537–538
89. Sune D, Rydberg H, Augustinsson ÅN, Serrander L, Jungeström MB 2020. Optimization of 16S rRNA gene analysis for use in the diagnostic clinical microbiology service. J. Microbiol. Methods 170: 105854.
90. Tan SY and McCoy AN 2020. James Dewey Watson (1928–): Co-discoverer of the structure of DNA. Singapore Med J. 61(10): 507–508. doi: 10.11622/smedj.2020145
91. Tantray J.A, Mansoor S, Wani R.FC, Nissa NU 2023. Chapter 24—Agarose gel electrophoresis. In Basic Life Science Methods; Academic Press: Cambridge, MA, USA: 103–106.
92. Tomioka Y, Arakawa T, Akuta T, Nakagawa M, Ishibashi M 2022 . Analysis of proteins by agarose native gel electrophoresis in the presence of solvent additives. Int. J. Biol. Macromol, 198, 26–36.
93. Tourneau L , Christophe, Kamal, Maud 2015. Pan-cancer integrative molecular portrait towards a new paradigm in precision medicine. Springer.
94. Tran Q, Pham D, and Phan V 2017. Using 16S rRNA gene as marker to detectunknown bacteria in microbial communities. BMC Bioinformatics, 18(14):499.
95. Venegas CN, and Donoso PC 2022. Some details in the study of viruses that affect to small animals. GSC Advanced Research and Reviews, 10(03): 019–024.https: //doi.org/ 10.30574/gscarr.2022.10.3.0068
96. Verma A, Sampla AK, and Tyagi JS 1999. Mycobacterium tuberculosis promoters: differential usage and growth rate dependent control. J Bacteriol 181:4326–4333
97. Watson JD, Crick FH 1953. Molecular structure of nucleic acids: A structure for deoxyribose nucleic acid. Nature 171: 737-738.
98. Westermeier R and Freiburg 2005. Gel Electrophoresis. ENCYCLOPEDIA OF LIFE SCIENCES, John Wiley & Sons, Ltd. www.els.net doi: 10.1038/npg.els.0005335
99. Wikström M, Krab K and Sharma V 2018. Oxygen Activation and Energy Conservation by Cytochrome c Oxidase. Chem. Rev. 118: 2469−2490
100. Wilson ML, Fleming KA, Kuti MA, Looi LM, Lago N, Ru K 2018. Access to pathology and laboratory medicine services: A crucial gap. Lancet 391, 1927–1938.
101. Wittmeier P, and Humme S 2022. Agarose gel electrophoresis to assess PCRproduct yield: comparison with spectrophotometry, fluorometry and qPCR. BioTechniques 72: 155–158.10.2144/btn-2021-0094
102. Yuan Hu 2016. Regulatory Concern of Polymerase Chain Reaction (PCR) Carryover Contamination .http: //dsx. doi. org/10.5772/66294
103. Lei, Z., Alwan, M., Alamir, H. T. A., Alkaaby, H. H. C., Farhan, S. S., Awadh, S. A., ... & Nekuei, A. (2022). Detection of abemaciclib, an anti-breast cancer agent, using a new electrochemical DNA biosensor. Frontiers in Chemistry, 10, 980162.
104. Bashar, B. S., Kareem, H. A., Hasan, Y. M., Ahmad, N., Alshehri, A. M., Al-Majdi, K., ... & Qasim, M. T. (2022). Application of novel Fe3O4/Zn-metal organic framework magnetic nanostructures as an antimicrobial agent and magnetic nanocatalyst in the synthesis of heterocyclic compounds. Frontiers in Chemistry, 10, 1014731.
105. Qasim, M. T., Fenjan, M. N., & Thijail, H. A. (2022). Molecular identification of cystoisospora belli in patients infected with the virus human immunodeficiency. International Journal of Drug Delivery Technology, 12(2), 701-704.
106. Al-Hchaimi, H. A., Alhamaidah, M. F., Alkhfaji, H., Qasim, M. T., Al-Nussairi, A. H., & Abd-Alzahra, H. S. (2022, October). Intraoperative Fluid Management for Major Neurosurgery: Narrative study. In 2022 International Symposium on Multidisciplinary Studies and Innovative Technologies (ISMSIT) (pp. 311-314). IEEE.
107. Mohammed, Z., & Qasim, M. T. (2022). The Relationship between Insulin Resistance and Hypertension in Patient with Hypertensive. Hiv Nursing, 22(2), 1659-1663.
108. Lafta, H. A., AbdulHussein, A. H., Al-Shalah, S. A., Alnassar, Y. S., Mohammed, N. M., Akram, S. M., ... & Najafi, M. (2023). Tumor-Associated Macrophages (TAMs) in Cancer Resistance; Modula-tion by Natural Products. Current topics in medicinal chemistry.
2. Abd al-Rida AA, Yasir I K, Swadi A J 2019. The bacteriological study of Acinetobacterbaumannii isolated from burn patients in al-Nasiriyah city-Iraq. University of Thi-Qar Journal 14(4):26.
3. Aguilera‑Arreola MG, Martínez‑Peña MD, Hernández‑Martínez F, Enriques SRJ, Verdín BR, Majalca‑Martínez C, Castro‑Escarpulli G, Albarrán‑Fernández E and CeSerrano‑López S C 2016.Cultivation‑independent approach for the direct detection of bacteria in human clinical specimens as a tool for analyzing culture‑negative samples: a prospective study.SpringerPlus,5:332
4. Al-Hilali R and Al-Mozan H 2023. Isolation and identification of Gram negative bacteria that cause diarrhea. University of Thi-Qar Journal of Science 10(1):1-6.
5. AL-Joda BMS and Jasim AH 2021. Biochemical Testing Revision For Identification Several Kinds of Bacteria. Journal of University of Babylon for Pure and Applied Science, 29(2): 168-176.
6. Al-Mozan HDK 2021. Molecular detection of enterotoxins genes in Staphylococcusaureus isolated from clinical and dairy samples. A thesis submitted to the college of science/ University of Basrah:1-131.
7. Al-Mozan HDK and Al-Amara SSM 2020. The nuc gene as a potent source for Staphylococcus aureus to resist thermal processes. International Journal of Pharmaceutical Research 12(2): 4614-4617.
8. Al-Yousif N A 2022. Distribution, occurrence and molecular characterization of Bacillus related species isolated from different soil in Basrah Province, Iraq. Biodiversitas Journal of Biological Diversity 23(2):679-686.
9. Aminollah BI, and Supriyanto A (2016). Isolasi dan Identifikasi Bakteri Patogen Escherichia coli Dan Salmonella sp pada Kotoran Kelelawar di Gua Pongangan, Gresik dan Gudang Talun Bojonegoro, Jawa Timur, in: Prosiding Seminar Nasional. Surabaya. Available at: http://biologi.fst.unair.ac.id/wp-content/uploads/2016/10/Aminollah.pdf
10. Amutha K, and Kokila V 2014. PCR Amplification, Sequencing of 16S rRNA Genes with Universal Primers and Phylogenetic Analysis of Pseudomonas aeruginosa. International Journal of Science and Research, 3(8):257-261.
11. Anahtar MN, Bowman BA, Kwon DS 2016. Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization. Journal of Visualized Experiments, (110), e53939, doi:10.3791/53939
12. Aryal, S. (2018a). Methyl Red (MR) Test - Principle, Procedure and Result Interpretation.https://microbiologyinfo.com/methyl-red-mr-test-principle-procedure-and-result-interpretation/
13. Aryal, S. (2018b). Voges-Proskauer (VP) Test - Principle, Reagents, Procedure and Result.https://microbiologyinfo.com/voges-proskauer-vp-test-principle-reagents-procedure-and-result/
14. Ayeni FA, Gbarabon T, Andersen C, Nørskov-Lauritsen N. 2015. Comparison of identification and antimicrobial resistance pattern of Staphylococcus aureus isolated from Amassoma, Bayelsa state, Nigeria. African Health Sciences 15(4):1282-1288. DOI10. 4314/ ahs. v15i4. 30.
15. Badreh F, Jahanbin K, Khodadadi A, Zadeh AK, Sharifat M, Khayati M, Rashno M 2022. Homemade 100 Pair Base DNA Ladder Based on Bacterial Plasmid pMAL-C2X. Pharmacogenom Pharmacoproteomics, 14 (3): 1-4.
16. Bailen M, Bressa C, Larossa M, and Gonzales-Sontero R. 2020. Bioinformatic strategies to address limitations of 16rRNA short-read amplicons from different sequencing platforms. Journal of Microbiological Method, 169, 105811.
17. Beden MW and Mousa H M 2022. Bacteriuria with Unusual Uropathogens in Diabetics in Thi-Qar Province. University of Thi-QarJournal of Science 9(1):43-48.
18. Benson DA. et al. 2013. GenBank. Nucleic Acids Res. 41, D36–D42.
19. Burillo A and Bouza E 2014. Use of rapid diagnostic techniques in ICU patients with infections. BMC Infectious Diseases 14:1-12.
20. Cahyadi M i, Puruhita, Barido F H, and Hertanto B S 2017. Specific primer design of mitochondrial 12S rRNA for species identification in raw meats. IOP Conf. Series: Earth and Environmental Science 102,012038.doi:10.1088/17551315/102/1/012038
21. Ceyssens P, Glonti T, Kropinski NM, Lavigne R, Chanishvili N, Leonid Kulakov L, Nino Lashkhi N, Tediashvili M and Merabishvili M 2011. Phenotypic and genotypic variations within a single bacteriophage species. Virology Journal, 8: 134. http:// www. virologyj. com/ content/ 8/1/134
22. Chen L, Cai Y, Zhou G, Shi X, Su J, Chen G and Lin K 2014. Rapid Sanger Sequencing of the 16S rRNA Gene for Identification of Some Common Pathogens. PLoS One. 9(2): e88886 doi: 10.1371/journal.pone.0088886
23. Chiu CY and Miller SA 2019. Clinical metagenomics. Nat. Rev. Genet. 20: 341–355.
24. Clarridge JE 2004. Impact of 16S rRNA Gene Sequence Analysis for Identification of Bacteria on Clinical Microbiology and Infectious Diseases. CLINICAL MICROBIOLOGY REVIEWS 17(4): 840–862.
25. Das S, Dash HR, Mangwani N, Chakraborty J and Kumari S 2014. Understanding molecular identification and polyphasic taxonomic approaches for genetic relatedness and phylogenetic relationships of microorganisms. Journal of Microbiological Methods 103: 80–100.
26. Davis NM, Proctor DM, Holmes SP, Relman DA and Callahan BJ 2018. Simple statistical identification and removal of contaminant sequences in marker-gene and metagenomics data. Microbiome, 6:226. https://doi. org/10. 1186/ s40168-018-0605-2
27. Desjardins RP, Conklin DS 2011. Microvolume quantitation of nucleic acids. Curr. Protoc. Mol. Biol. 93(1), 3J.
28. Devlynne S, Ondusko MD, Dawn Nolt, MD 2018.Staphylococcusaureus. Oregon Health & Science University, Portland, 39(6):287-298.
29. Dhahir, A.W. (2023). Genotypic Characterization of Bacteria isolated from intensive care units in Basrah hospitals. A thesis submitted to the council of college of science, university of Basrah.
30. Dickson R P et al. 2020. Lung microbiota predict clinical outcomes in critically Ill patients. Am. J. Respir. Crit. Care Med. 201(5), 555–563.
31. Edgar RC 2018. Updating the 97% identity threshold for 16S ribosomal RNA OTUs. Bioinformatics, 34(14),: 2371–2375. doi: 10.1093/bioinformatics/bty113
32. ELMA LL, ALBERTO F, DE OLIVEIRA JR, FILLIPE L.R, CARMO D,BERKOVA N, BARH D, PREETAM GHOSH P, and AZEVEDO V 2020. Bacteriocins as an alternative in the treatment of infections by Staphylococcus aureus. An Acad Bras Cienc , 92(2):1-17.
33. Ernande B and Dieckmann U 2004. The evolution of phenotypic plasticity in spatially structured environments: implications of intraspecific competition, plasticity costs and environmental characteristics. J . EVOL. B IOL. 17: 613–628.
34. Favrot C 2015. Polymerase chain reaction: advantages and drawbacks. Posted at the Zurich Open Repository and Archive, University of Zurich ZORA URL: https://doi.org/10.5167/uzh-116536
35. Fernández-Barat L, López-Aladid R. and Torres A 2020. Reconsidering ventilator-associated pneumonia from a new dimension of the lung microbiome. EBioMedicine 60, 102995.
36. Gee JE, De BK, Levett PN, Whitney AM Novak RT and Popovic T 2004.Rapid Confirmatory Identification of Use of 16S rRNA Gene Sequencing for Brucella Isolates. J. Clin. Microbiol. 2004, 42(8):3649. DOI:10.1128/JCM.42.8.3649-3654.2004.
37. Ghannam MG, and Varacallo M 2023. Biochemistry, Polymerase Chain Reaction.National Library of Medicine, https://www.ncbi.nlm.nih.gov/books/NBK535453/
38. Gomez-Mestre I and Jovani R. 2013. A heuristic model on the role of plasticity in adaptive evolution: plasticity increases adaptation, population viability and genetic variation. Proceedings of the Royal Society B 280:20131869
39. Goswami K, Chen C, Xiaoli L, Eaton KA Dudley EG 2015. Coculture of Escherichiacoli O157: H7 with a Nanpathogenic E. coli strain increases toxin production and virulence in a germfree mouse model. Infection and Immunity 83(11): 4185- 4193.
40. Graham S, Marina PF, Blencowe A. 2019. Thermoresponsive polysaccharides and their thermoreversible physical hydrogel networks. Carbohydr. Polym. 207, 143–159.
41. Gu W, Miller S, Chiu CY 2019. Clinical Metagenomic Next- Generation Sequencing for Pathogen Detection.Annu. Rev. Pathol.14: 319–338.
42. Haines AM, Tobe S, Kobus HJ, Linacre A 2015. Properties of nucleic acid staining dyes used in gel electrophoresis. Electrophoresis 36(6): 941–944.
43. Hartline, R 2023.MICROBIOLOGY LABORATORY MANUAL. LabreTexts.https://LibreTexts.org
44. Heather JM, and Chain B 2015. The sequence of sequencers: The history of sequencing DNA. Genomics 107: 1–8. http://dx.doi.org/10.1016/j.ygeno.2015.11.003.
45. Heckmann CM and Paradisi F 2020. Looking Back: A Short History of the Discovery of Enzymes and How They Became Powerful Chemical Tools. ChemCatChem , 12: 6082–6102. doi.org/10.1002/cctc.202001107
46. Hemraj V, Diksha S and Avneet G 2013. A REVIEW ON COMMONLY USED BIOCHEMICAL TEST FOR BACTERIA. Innovare Journal of Life Science, 1(1):1-7.
47. Hendry AP 2015. Key questions on the role of phenotypic plasticity in eco evolutionary dynamics. Journal of Heredity 107:25–41.
48. Hunt K, Vacelet M and Jordan K 2017.Determination of Listeria monocytogenes numbers at less than 10 cfu/g. Irish Journal of Agricultural and Food Research,DOI: 10.1515/ijafr-2017-0004 IJAFR • 56(1) :25-30.
49. Igbeneghu O and Lamikanra A 2014. The bacteriological quality of different brands of bottled water available to consumers in Ile-Ife, south- western Nigeria. a BMC Research Notes 7:859.
50. Irwan JM, Anneza LH, Othman N, Husnul T and Alshalif AF 2016. Isolation and Identification of Concrete Environment Bacteria. IOP Conf. Series: Materials Science and Engineering 136, 012088 doi:10.1088 /1757-899X/136/1/012088
51. Ishino S, and Ishino Y 2014. DNA polymerases as useful reagents for biotechnology – the history of developmental research in the field, Front Microbiol. 2014; 5: 465. doi: 10.3389/fmicb.2014.00465
52. Jang J , Hur HG, Sadowsky MJ, Byappanahalli MN, Yan T and Ishii S 2017. Environmental Escherichia coli: ecology and public healthimplications—a review. Journal of Applied Microbiology, 123, 570—581.
53. Jeong T, Hong H, Kim MS, Song M, and Myung H 2023. Characterization of Three Different Endolysins Effective against Gram-Negative Bacteria. Viruses, 15, 679. https://doi.org/10.3390/v15030679.
54. Jesumirhewe C J, Ogunlowo PO, Olley M, Springer B, Allerberger F and Ruppitsch W 2016. Accuracy of conventional identification methods used for Enterobacteriaceae isolates in three Nigerian hospitals. PeerJ, DOI 10.7717/peerj.2511
55. Jiang F, Xu X, Chen F, Weng H, Jun Chen J, Ru Y, Xiao Q and Xiao A 2023. Extraction, Modification and Biomedical Application of Agarose Hydrogels: A Review. Mar. Drugs , 21: 299 .https://doi.org/10.3390/md21050299
56. Joshi, M and Deshpande J.D 2011. POLYMERASE CHAIN REACTION: METHODS, PRINCIPLES AND APPLICATION. International Journal of Biomedical Research, 1(5): 81‐97.
57. Karnath L 2019. EDITORIAL Remembering Dr. Kary Mullis— Inventor of PCR. MOLECULAR FRONTIERS JOURNAL, 3(2).
58. Kaushal J, Mehandia S, Singh G, Raina A, Arya S K.2018. Catalase enzyme: Application in bioremediation and food industry. Biocatalysis and Agricultural Biotechnology, 16: 192-199.
59. Kchouk M, Gibrat J and Elloumi M 2017. Generations of Sequencing Technologies: From First to Next Generation. Biol Med, 9 (3): 1-8.
60. Kshikhundo R, and Itumhelo S 2016. Bacterial species identification. World News of Natural Sciences, 3: 26-38.
61. Lakshmi P, Bharadwaj A and Srivastava RK 2020.Molecular Detection and Identification of Bacteria in Urine Samplesof Asymptomatic and Symptomatic PregnantWomen by 16S Rrna Gene Sequencing.Arch Clin Infect Dis. 15(3) :e101136.doi:10.5812/archcid.101136.
62. Leraya M, Knowltonb N, Hoc S, Bryan N. Nguyenb BN, and Machidac RJ 2019. GenBank is a reliable resource for 21st century biodiversity research. ENVIRONMENTAL SCIENCES, 116(45): 22651–22656. www.pnas. org/cgi/ doi/ 10. 1073/pnas.1911714116
63. Li C and Arakawa T 2019. Agarose native gel electrophoresis of proteins. Int. J. Biol. Macromol, 140: 668–671.
64. Lindner MS, Renard BY 2013. Metagenomic abundance estimation and diagnostic testing on species level. Nucleic Acids Res4, 1(1):10–10.
65. Lira RB, Steinkuhler J, Knorr R.L, Dimova R, Riske KA 2016. Posing for a picture: Vesicle immobilization in agarose gel. Sci. Rep. 6: 25254.
66. Mai-Prochnow A, Clauson M, Hong, J and Murphy AB 2016. Gram positive and Gram negative bacteria differ in their sensitivity to cold plasma. Scientific Reports | 6:38610 | DOI:10.1038/srep38610
67. Mardis E, McCombie WR 2017. Library quantification: fluorometric quantitation of double-stranded or single-stranded DNA samples using the Qubit system. Cold Spring Harb. Protoc. (6),pdb.prot094730
68. Marx V 2016. PCR: the price of infidelity. Nature Methods. U.S. Food & Drug Administration, 13:475–479. DOI: 10.1038/nmeth.3868
69. Maurer FM, Christner M, Hentschke M, Rohde H 2017. Advances in rapid identification and susceptibility testing of bacteria in the clinical microbiology laboratory: implications for patient care and antimicrobial stewardship programs. Infectious Disease Reports 9:18-27.
70. Muktaf A Q 2022. Effect of hot aqueous and ethanolic extracts of two algae species (Phaeophyta) on some pathogenic bacteria isolated from patients. A thesis submitted to the college of science/ University of Thi-Qar:1-118.
71. Murren CJ, Auld JR, Callahan H, Ghalambor CK, Handelsman CA, Heskel MA, Kingsolver JG, Maclean HJ, Masel J, MaughanH, Pfennig DW, Relyea RA, Seiter S, Snell-Rood E, Steiner UK and Schlichting CD 2015. Constraints on the evolution of phenotypic plasticity: limits and costs of phenotype and plasticity. Heredity, 115, 293–301; doi: 10. 1038/ hdy. 2015.8
72. Nagai T, Shiba T, Komatsu K, Watanabe T, Nemoto T, Maekawa S, Kobayashi R, Matsumura S, Ohsugi Y, Katagiri S, Takeuchi Y, Iwata T 2023. Optimal 16S rRNA gene amplicon sequencing analysis for oral microbiota to avoid the potential bias introduced by trimming length, primer, and database. DOI: https://doi.org/10.21203/rs.3.rs-3139837/v1
73. Negahdaripour M and PharmD 2020. Fifty Years of Witnessing Biomedical Science Developments. Iran J Med Sci November, 45 (6).
74. Petti C A, Polage C R, Schreckenberger P (2005). The Role of 16S rRNA Gene Sequencing in Identification of Microorganisms Misidentified by Conventional Methods. JOURNAL OF CLINICAL MICROBIOLOGY 43 (12): 6123–6125.
75. Porter TM and Hajibabaei M 2018. Over 2.5 million COI sequences in GenBank and growing. PLoS One 13, e0200177
76. Public Health England. 2018. UK Standards for Microbiology Investigations. Issued by the Standards Unit, Public Health England, 4:12
77. Putria I, Jannaha SN and Purwantisaria S 2020. Isolation and characterization of lactic acid bacteria from Apis mellifera and their potential as antibacterial using in vitro test against growth of Listeria monocytogenes and Escherichia coli.NICHE Journal of Tropical Biology, 3(1): 26-34.
78. Qazangi MO and Jabr AH 2017. Molecular Biology. 1th Edition, Iraq, University of Baghdad – Science of Agriculture, 1-436.
79. Qazangi MOM 2017.Practical experiments in microbiology. 1th Edition, Iraq, University of Baghdad – Science of Agriculture : 1-138.
80. RASHEED MU, THAJUDDIN N. AHAMED P, TEKLEMARIAM Z, and JAMIL K 2014. ANTIMICROBIAL DRUG RESISTANCE IN STRAINS OF Escherichia coli ISOLATED FROM FOOD SOURCES. Med. Trop. Sao Paulo, 56(4): 341-6.
81. Reddy PR and Raju N (2012). Gel-Electrophoresis and Its Applications, Gel Electrophoresis - Principles and Basics, Sameh Magdeldin , ISBN: 978-953-51-0458-2, InTech, Available from: http://www.intechopen.com/books/gel-electrophoresis-principles-and-basics/ gelelectrophoresi-andits applications
82. Rizal NSM., Ramli R., Periyasamy PR, Tan TL, Nathan S, Saw SH, Khor BY 2020. Advantages and Limitations of 16S rRNA Next-Generation Sequencing for Pathogen Identification in the Diagnostic Microbiology Laboratory: Perspectives from a Middle-Income Country. Diagnostics 816: 1-19. doi:10.3390/diagnostics10100816
83. Romaniuk J A. H. and Cegelski L 2015. Bacterial cell wall composition and the influence of antibiotics by cell-wall and whole-cell NMR. the Royal Society, http://dx.doi.org/10.1098/rstb.2015.0024
84. Shanmugaraj C, Anokhe A, and Kalia V 2021. Determination of Fermentation Pathway by Methyl Red and Voges Proskauer (MRVP) Test. AgriCos e-Newsletter,2(11):41-43.
85. Shepherd M.J, Horton J.S, and Taylor T.B 2022. A Near-Deterministic Mutational Hotspot in Pseudomonas fluorescens Is Constructed by Multiple Interacting Genomic Features. Mol. Biol. Evol. 39(6):1-7. https://doi.org/10.1093/molbev/msac132
86. Silhavy TJ, Kahne D, Walker S 2010. The bacterial cell envelope. Cold Spring Harbor Perspectives in Biology 2, a000414. doi: 10.1101/cshperspect.a000414.
87. Srinivasan R, Karaoz U, Volegova M MacKichan J, Kato- Maeda M, Miller S, Nadarajan R, Brodie EL and Lynch SV 2015. Use of 16S rRNA Gene for Identification of a Broad Range of Clinically Relevant Bacterial Pathogens. PLOS ONE | DOI:10.1371/journal.pone.0117617
88. Strasser B J 2008. GenBank—Natural history in the 21st century? Science 322: 537–538
89. Sune D, Rydberg H, Augustinsson ÅN, Serrander L, Jungeström MB 2020. Optimization of 16S rRNA gene analysis for use in the diagnostic clinical microbiology service. J. Microbiol. Methods 170: 105854.
90. Tan SY and McCoy AN 2020. James Dewey Watson (1928–): Co-discoverer of the structure of DNA. Singapore Med J. 61(10): 507–508. doi: 10.11622/smedj.2020145
91. Tantray J.A, Mansoor S, Wani R.FC, Nissa NU 2023. Chapter 24—Agarose gel electrophoresis. In Basic Life Science Methods; Academic Press: Cambridge, MA, USA: 103–106.
92. Tomioka Y, Arakawa T, Akuta T, Nakagawa M, Ishibashi M 2022 . Analysis of proteins by agarose native gel electrophoresis in the presence of solvent additives. Int. J. Biol. Macromol, 198, 26–36.
93. Tourneau L , Christophe, Kamal, Maud 2015. Pan-cancer integrative molecular portrait towards a new paradigm in precision medicine. Springer.
94. Tran Q, Pham D, and Phan V 2017. Using 16S rRNA gene as marker to detectunknown bacteria in microbial communities. BMC Bioinformatics, 18(14):499.
95. Venegas CN, and Donoso PC 2022. Some details in the study of viruses that affect to small animals. GSC Advanced Research and Reviews, 10(03): 019–024.https: //doi.org/ 10.30574/gscarr.2022.10.3.0068
96. Verma A, Sampla AK, and Tyagi JS 1999. Mycobacterium tuberculosis promoters: differential usage and growth rate dependent control. J Bacteriol 181:4326–4333
97. Watson JD, Crick FH 1953. Molecular structure of nucleic acids: A structure for deoxyribose nucleic acid. Nature 171: 737-738.
98. Westermeier R and Freiburg 2005. Gel Electrophoresis. ENCYCLOPEDIA OF LIFE SCIENCES, John Wiley & Sons, Ltd. www.els.net doi: 10.1038/npg.els.0005335
99. Wikström M, Krab K and Sharma V 2018. Oxygen Activation and Energy Conservation by Cytochrome c Oxidase. Chem. Rev. 118: 2469−2490
100. Wilson ML, Fleming KA, Kuti MA, Looi LM, Lago N, Ru K 2018. Access to pathology and laboratory medicine services: A crucial gap. Lancet 391, 1927–1938.
101. Wittmeier P, and Humme S 2022. Agarose gel electrophoresis to assess PCRproduct yield: comparison with spectrophotometry, fluorometry and qPCR. BioTechniques 72: 155–158.10.2144/btn-2021-0094
102. Yuan Hu 2016. Regulatory Concern of Polymerase Chain Reaction (PCR) Carryover Contamination .http: //dsx. doi. org/10.5772/66294
103. Lei, Z., Alwan, M., Alamir, H. T. A., Alkaaby, H. H. C., Farhan, S. S., Awadh, S. A., ... & Nekuei, A. (2022). Detection of abemaciclib, an anti-breast cancer agent, using a new electrochemical DNA biosensor. Frontiers in Chemistry, 10, 980162.
104. Bashar, B. S., Kareem, H. A., Hasan, Y. M., Ahmad, N., Alshehri, A. M., Al-Majdi, K., ... & Qasim, M. T. (2022). Application of novel Fe3O4/Zn-metal organic framework magnetic nanostructures as an antimicrobial agent and magnetic nanocatalyst in the synthesis of heterocyclic compounds. Frontiers in Chemistry, 10, 1014731.
105. Qasim, M. T., Fenjan, M. N., & Thijail, H. A. (2022). Molecular identification of cystoisospora belli in patients infected with the virus human immunodeficiency. International Journal of Drug Delivery Technology, 12(2), 701-704.
106. Al-Hchaimi, H. A., Alhamaidah, M. F., Alkhfaji, H., Qasim, M. T., Al-Nussairi, A. H., & Abd-Alzahra, H. S. (2022, October). Intraoperative Fluid Management for Major Neurosurgery: Narrative study. In 2022 International Symposium on Multidisciplinary Studies and Innovative Technologies (ISMSIT) (pp. 311-314). IEEE.
107. Mohammed, Z., & Qasim, M. T. (2022). The Relationship between Insulin Resistance and Hypertension in Patient with Hypertensive. Hiv Nursing, 22(2), 1659-1663.
108. Lafta, H. A., AbdulHussein, A. H., Al-Shalah, S. A., Alnassar, Y. S., Mohammed, N. M., Akram, S. M., ... & Najafi, M. (2023). Tumor-Associated Macrophages (TAMs) in Cancer Resistance; Modula-tion by Natural Products. Current topics in medicinal chemistry.
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Review: Gene of 16S rRNA is the gold standard to diagnose bacteria. World Journal of Current Med and Pharm Research [Internet]. 2023 Aug. 27 [cited 2026 Jan. 27];5(4):139-78. Available from: https://www.wjcmpr.org/index.php/journal/article/view/283
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Review: Gene of 16S rRNA is the gold standard to diagnose bacteria. World Journal of Current Med and Pharm Research [Internet]. 2023 Aug. 27 [cited 2026 Jan. 27];5(4):139-78. Available from: https://www.wjcmpr.org/index.php/journal/article/view/283
